Coding

Part:BBa_K4482005

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-08-25)


NTRK3 eGFP

This part is a fusion protein of NTRK3 (BBa_K4482002) with eGFP at the C-terminus of NTRK3. The part was designed in order to identify cells expressing NTRK3 by fluorescent microscopy.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1840
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
    Illegal NgoMIV site found at 134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2483
    Illegal SapI site found at 435
    Illegal SapI site found at 1089


[edit]
Categories
Parameters
None